Development of Fluorescence Computational Algorithm for DNA Amplification

According to the increasing complexity of diagnosis, the current real-time polymerase chain reaction (PCR) platforms are equipped with highly developed analyzing capability. However, the sight biological signals are always hard to extract from the detected information. On the other hand, the current signal processing scheme makes the optical loss worse if there are more than four DNA labeling dyes. The proposed computational algorithm for the fluorescence detection system composes of a strong noise reduction mechanism to enhance the detection sensitivity. The whole system includes an ultra-sensitive spectrometer providing continuous wavelength detection and can be employed for multiple DNA quantification. The results show that this prototype provides comparable sensitivity and amplification efficiency as Roche Light-Cycler for DNA quantification and similar reproducibility within five intra assay samples with the DNA quantification method proposed in this study. Key-Words: Real-Time PCR, DNA Computation, Algorithm Realization.